Figure 6
Semiq-RT-PCR analysis of some ZmPME/PMEI genes in the nine different tissues of B73 inbred line and in the anthers of three fertile and three sterile individuals of a maize backcross population. (a) The total RNA of nine tissues including seedling roots, seedling leaves, 2-cm immature ears, non-emerged immature tassels, anthers, pollen, whole seeds after pollination for 18 days, endosperm and embryo after pollination for 20 days of B73 inbred line was isolated and used to perform the semiq-RT-PCR of the ZmPME/PMEI genes. (b) The total RNA of anthers of three sterile and three fertile individuals was isolated and used to perform the semiq-RT-PCR of the ZmPME/PMEI genes. Beta actin was used for internal controls to normalize the RNA contents in each sample. Primers used are shown in Supplementary Table 5. In the figure, the PCR products were separated with the same experiment condition and that gels were processed in parallel. The original gels were presented in Supplementary Fig. S11.
