Figure 5
From: High resolution structural and functional analysis of a hemopexin motif protein from Dolichos

Binding affinity of DC25 with TPPS4, and induced-fit docking. (a) Analysis of DC25 (ligand) binding affinity with different concentrations of TPPS4 (analyte) in range of 0.1 to 12.5 µM by SPR. RU, response units. (b) DC25 has been shown in sky blue ribbon whereas docked TPPS4 has been shown in a light pink stick. The H-bond interactions (magenta dash lines) between amino acid residues (green) and -SO42− (yellow) of TPPS4. (c) Electrostatic potential surface view of the docked TPPS4 on DC25. The figure was prepared using PyMOL26 (The PyMOL Molecular Graphics System; http://www.pymol.Org).