Figure 1 | Scientific Reports

Figure 1

From: Reversibility of membrane permeabilization upon pulsed electric field treatment in Lactobacillus plantarum WCFS1

Figure 1

Schematic overview of the two staining methods used in this study. Dashed black lines represent damaged/permeabilized membranes and intact black lines represent intact cell membranes. In method 1 a mixture of PI (red) and counterstain SYTO 9 (green) was added either before PEF treatment (A) or after PEF treatment (B). Reversibility of membrane integrity is based on the difference in PI uptake between A and B. In method 2, the PI stain (red) was added before PEF and SYTOX Green (green) after the PEF treatment. Reversibility of membrane integrity is based on PI and/or SYTOX Green uptake. Coloured squares above the arrows indicate the moment in the procedure when the stain(s) were added to the culture.

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