Figure 4

Relative mean forward scatter (FSC) signals for three different fractions of the population; unaffected intact cells, reversible permeabilized cells and irreversible permeabilized cells. The different groups are determined based on PI and SYTOX Green staining and relative mean FSC values are determined by dividing the mean FSC signals of specific populations by the mean FSC value of the entire cell population. The data point presented at 0 kV/cm is a control; namely the mean FSC of the control culture divided over the mean FSC of the PEF treated cultures. Error bars represent standard deviations of biologically independent duplicates and different letters indicate significant differences (P < 0.05) between the three fractions at one specific electric field strength.