Table 1 Determination of the unaffected, reversible, irreversible and initially permeabilized fractions of the cell population upon PEF treatment as analysed by the two different fluorescence staining methods.

From: Reversibility of membrane permeabilization upon pulsed electric field treatment in Lactobacillus plantarum WCFS1

 

Method 1

PI before and after PEF with counterstain SYTO 9

Method 2

PI before and SYTOX Green after PEF

Not permeabilized by PEF (unaffected, intact membrane)

% green cells in sample stained before PEF

% cells low in PI and low in SYTOX Green fluorescence

Reversible permeabilized cells

% green cells in sample stained after PEF minus % green cells in sample stained before PEF

% cells high in PI, low in SYTOX Green fluorescence

Irreversible permeabilized cells

% green cells in control sample minus % green cells in sample stained after PEF

% cells high in PI and high in SYTOX Green fluorescence

Cells already permeabilized before PEF

100% minus green cells in control sample (no PEF)*

n.a*

  1. *The fraction of cells already permeabilized before PEF was checked for every experiment in a control sample and was <2% for all cultures.
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