Figure 2 | Scientific Reports

Figure 2

From: Mycobacterium tuberculosis clinical isolates of the Beijing and East-African Indian lineage induce fundamentally different host responses in mice compared to H37Rv

Figure 2

Lymphoid cell populations in the lungs of mice infected with different Mtb strains. Lymphoid cells were determined in the lungs of mice infected with Beijing-1585 (black bars), EAI-1627 (open bars) or H37Rv (grey bars) and compared to uninfected control mice (HC, striped bars). Gating strategies are shown in Fig. S5. (A) B-cells, identified based on scatterplot and high level CD45R expression, are significantly higher in number for Beijing-1585- and EAI-1627-infected mice at 14 dpi compared with H37Rv at 14 and 28 dpi. (B,C) Only H37Rv infection induces an increase in both CD4+ and CD8+ T-cells at 28 dpi. (D) Despite the increase in T-cells caused by H37Rv infection at 28 dpi, Foxp3+ regulatory T-cells are significantly lower compared to Beijing-1585- and EAI-1627-infected mice at 14 dpi. Gating strategies were similar as described previously51. N = 6 mice per group per time point, *p < 0.05, **p < 0.01, ***p < 0.001 after Bonferroni correction. Data are shown as % of total lung single cell suspension.

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