Figure 4

Myeloid cell populations in the lungs of mice infected with different Mtb strains. CD11b⁺ cells were classified as PMN (CD11b⁺Ly6G^high), iM/DC (CD11b⁺Ly6C^intCD11c^high), monocyte-like cells (Mo-like) (CD11b⁺Ly6C^highCD11c^low) and alveolar macrophages (AM) (CD11b^intCD11c^highSiglec-F⁺) in the lungs of mice infected with Beijing-1585 (black bars), EAI-1627 (open bars) or H37Rv (grey bars) compared to uninfected control mice (HC, striped bars). Gating strategies are shown in Fig. S5. (A) PMN cells showed a more rapid increase for Beijng-1585 and EAI-1627 compared to H37Rv. (B) iM/DC showed kinetics comparable to PMN for the different groups. (C) Monocyte-like cells are only higher in the EAI-1627 group at 14 dpi compared to the H37Rv group at 28 dpi. (D) Lung alveolar macrophages are lower in the Beijing-1585 and EAI-1627 group compared to the H37Rv group at 14 dpi. N = 6 mice per group per time point, *p < 0.05, **p < 0.01, ***p < 0.001 after Bonferroni correction.