Figure 6 | Scientific Reports

Figure 6

From: Mycobacterium tuberculosis clinical isolates of the Beijing and East-African Indian lineage induce fundamentally different host responses in mice compared to H37Rv

Figure 6

Cytokine mRNA expression levels in bone marrow of mice infected with different Mtb strains. Expression levels of target cytokine mRNA are shown relative to Gapdh in the bone marrow of mice infected with Beijing-1585 (black bars), EAI-1627 (open bars) or H37Rv (grey bars). (A) IL-12p35 expression at 3 dpi and 7 dpi was lower in the Beijing-1585 group compared to H37Rv. (B) IFN-γ expression was lower for Beijing-1585 at 3 dpi compared to H37Rv. (C) IL-17a levels were markedly lower in the Beijing-1585 group compared to the H37Rv group (mean of 0.26 vs. 26.7), but without statistical significance (p = 0.06 after Bonferroni correction) due to the high spread in the H37Rv group (5.3–71.8). (D) TNF-α levels were higher in the bone marrow of H37Rv-infected mice compared to Beijing-1585-infected mice at all time points evaluated. EAI-1627 consistently showed intermediate results between Beijing-1585 and H37Rv for all cytokines. N = 6 mice per group per time point, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 after Bonferroni correction.

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