Figure 6 | Scientific Reports

Figure 6

From: Assessment of the hepatoprotective effect of developed lipid-polymer hybrid nanoparticles (LPHNPs) encapsulating naturally extracted β-Sitosterol against CCl4 induced hepatotoxicity in rats

Figure 6

Immunostaining for cleaved caspase-3 protein in liver sections of rats, (a) normal control group showing no cleaved caspase-3 immune-reactive cells. (b) Treated with CCl4 showing strong positive immune expression (arrow). (c) Co-treated with CCl4 + BSS suspension showing strong positive immune reactive hepatocytes (arrow). (d) Co-treated with CCl4 + plain LPHNPs formula, showing moderate positive immune expression (arrow). (e) Co-treated with CCl4 + BSS-LPHNPs (200 mg/kg), (f) co-treated with CCl4 + BSS-LPHNPs (400 mg/kg), (e,f) showing weak positive cleaved caspase-3 reaction (arrow). (g) Immunostaining area (%) of cleaved caspase-3 expression. Data shown as mean ± SE; error bars show the variations of determinations in terms of standard errors, mean values with unlike superscript letters were significantly different(p ≤ 0.05).

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