Figure 1
From: Selection and functional identification of a synthetic partial ABA agonist, S7
Construction of an ABA-hypersensitive synthetic promoter and its use for the identification of an ABA mimic in vivo chemical screen. (A) The synthetic promoter constructs and the cis-elements they contain. (B) Luciferase (LUC) assay of the ABA sensitivity of several synthetic promoters in rice protoplasts. (C) Representative chemical screening results using the in vivo screening system for factors regulating ABA signalling in rice protoplasts. (D) Comparison of S7 activity in ABA-dependent and ABA-independent signalling, performed using the ABA-sensitive promoter pD2-2 × DRE and the ABA-insensitive promoter pD2, respectively. (E) The two-dimensional structures of S7 and ABA. For the LUC assays, each construct harbouring the indicated promoter was introduced into rice protoplasts using the PEG-mediated method. After incubation for 15 h with the indicated chemicals, the LUC activity was detected. The values are the average of three replications and were normalized relative to the control. Error bars indicate ± SD.
