Figure 3
From: Selection and functional identification of a synthetic partial ABA agonist, S7
S7 mediated interaction between OsPYLs and OsPP2CAs. (A) Yeast two-hybrid assay. The droplets on the left were produced using a 1:10 dilution of yeast grown to saturation in SD (−) LT, while those on the right were diluted 1:100. (B) Pull-down assay of GST-tagged proteins using GSH-conjugated agarose beads. The proteins were detected using a western blot analysis with anti-His mouse antibodies and anti-GST rabbit antibodies. The full length blot is presented in Supplementary Fig. 4.
