Figure 5
From: Spatio-temporal expression and distribution of collagen VI during zebrafish development

ColVI expression in adult zebrafish tissues. (a) qRT-PCR for col6a1, col6a2, col6a3, col6a4a and col6a4b transcripts in different tissues of adult (8- to 12-mpf) fish. Data were normalized to eif1axb expression. Error bars indicate s.e.m. (b) Western blotting for α1(VI) chain in protein extracts of adult (8- to 12-mpf) fish tissues. Vinculin was used as a loading control. Number on the left indicate sizes (in kDa) of protein standard markers. The cropped blots for α1(VI) chain and vinculin derive from different parts of the same gel.