Figure 3
Response of the sensors to exogenously supplied metabolites. (a) Laconic FRET maps of wing imaginal discs incubated or not in 20 mM or 40 mM lactate for 15 min. Scale bar: 50 μm. Dotted lines mark the region in which the FRET signal was measured. (b) Quantification of the Laconic signal of the experiment of panel a. Each point represents the average value of a single imaginal disc. The grey area is limited by the SD of each data set. (c) Pyronic FRET signal from wing imaginal discs incubated or not with exogenous pyruvate for 15 min. Each point represents the average value of a single imaginal disc. The grey area is limited by the SD of each data set. (d) OGsor FRET signal from wing imaginal discs incubated or not with exogenous DM-2-OG for 15 min. Each point represents the average value of a single imaginal disc. The grey area is limited by the SD of each data set. (e) FRET maps of the indicated 3rd instar larval organs with or without addition of exogenous 40 mM lactate. Dotted lines mark the region in which the FRET signal was measured. Scale bar: 50 μm. (f) Quantification of the Laconic signal of the experiment shown in panel (e). Data represent the media +/− SD; p = 2.27E-08 for fat body, p = 1.0442E-09 for salivary gland, p = 3.596E-11 for brain; Student’s T-test; n ≥ 20 per group.
