Figure 6 | Scientific Reports

Figure 6

From: A genetic toolkit for the analysis of metabolic changes in Drosophila provides new insights into metabolic responses to stress and malignant transformation

Figure 6

Heterogeneity of the metabolic state of tumors with various genetic backgrounds. (a,b) Laconic FRET maps and quantification of tumors induced through the indicated genetic manipulation in wing discs, using an en-Gal4 driver. Scale bar: 50 μm. Data represent the media +/− SD. The FRET signal of each tumor sample was quantified in the total area of the disc, and then normalized to w RNAi control discs. The media of each condition was statistically compared with its own control data set; p = 3.7394E-05 for PVR, p = 3.482E-05 for Ras and p = 6.6187E-15 for l(2)gl RNAi, Student’s T-test; n ≥ 20 per group. (c) Summary of the lactate levels in each of the tumors. (d) Laconic FRET maps of tumors induced by l(2)gl silencing with an en-Gal4 driver. As time passes after the onset of l(2)gl RNAi expression, lactic acid gradually accumulates. (e) Quantification of the results shown in panel (d). Data represent the media +/− SD; * p < 0.05 Dunnet’s Test; n ≥ 20 per group.

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