Figure 3
Exchange rate constants, kHX, obtained by using the modified MEXICO NMR experiment monitoring potential exchange between amide protons and solvent protons taking place on a millisecond time scale. (A) Data are shown for residues comprising the proximal unit of artificially PA-linked Ub2s originating from Lys11- (colored in red) or Lys27-linkage type (colored in blue). (B) Differences of amide proton exchange rate constants, ΔkHX, comparing data presented in A to the exchange between amide protons and solvent protons taking place in monomeric wild type Ub. Secondary structural elements according to PDB ID 1D3Z are indicated on top.
