Figure 2
Self-assembly of β2-m D76N in transgenic C. elegans strain. (a) Immunoblot analysis of soluble (SF) and insoluble fraction (IF) of worm lysates grown at 25 °C resolved via 8–18% SDS PAGE and detected with anti-β2-m antibody (DAKO). The insoluble fraction was washed twice in PBS buffer before loading onto gel. (M = Molecular weight standard: Precision Plus Western C, BioRad). (b–d) Size-excluded soluble proteins from β2-m expressing nematodes and recombinant D76N β2-m. Shown is the absorbance at 280 nm (Abs 280) of eluted material against elution volume. Fractions (1 ml) were collected. (c–e) Immunoblot analysis of size-excluded fractions (6–20) of β2-m expressing worms’ lysates and recombinant β2-m resolved via 8–18% SDS PAGE and detected with anti-β2-m antibody (DAKO). Uncropped scans of immunoblots are shown in Supplementary Fig. S2a.
