Figure 5
Characterization of the phenotype of D76N β2-m strain by classic behavioral assays. (a) Egg-synchronized control worms (smg-1) and D76N β2-m expressing worms were placed at 23 °C into fresh NMG plates seeded with OP50 E. coli. At day 1, 5 and 6 of adulthood body bends were scored in liquid. At least three independent assays were performed. Data are mean of number of body bends/min ± SEM; **p < 0.01 and *p < 0.05 vs the control strain (smg-1), according to one-way ANOVA (N = 40 animals for each group). (b) Kaplan–Meier survival curves of control nematodes and D76N β2-m strain. Data are expressed as mean of three independent experiments (N = 40 animals for each group, χ2 = 5.52, p = 0.019 according to Peto-Peto-Prentice test). (c) Total eggs deposition for smg-1 and D76N β2m strains maintained at 23 °C. At least three independent assays were performed. Error bars represent the SEM, *p < 0.05 vs. the control strain (smg-1) according to t-test. (d) Percentage of not-hatched eggs of smg-1 and D76N β2-m strains analyzed 24 h after deposition. At least three independent assays were performed. Error bars represent the SEM, ***p < 0.001 vs. the control strain according to t-test.
