Figure 3
From: Lyse-Reseal Erythrocytes for Transfection of Plasmodium falciparum
(A) GFP expression in drug resistant parasites. P. falciparum 3D7 was transfected with pFCEN1 using LyRET and EiRBC methods. Drug resistant parasites were evaluated for GFP expression using live cell fluorescence microscopy. Shown are images for GFP expression (GFP), nuclear stain (Hoechst), bright field (DIC) and the overlap of all images (Merged). (B) The presence of HFDDI in parasites. P. falciparum 3D7 was transfected with HFDDI using LyRET and EiRBC methods. The presence of HFDDI in drug resistant parasites was determined by PCR amplification of P. yoelii α-tubulin region of the plasmid from the total DNA. The ethidium bromide stained agarose gel shows PCR products amplified from the total DNAs of parasites obtained by LyRET (lanes 3 and 5) and EiRBC (lanes 2 and 4) methods. Genomic DNA of wild type parasites served as a negative control (lane 1). The lane M contains DNA size markers with the positions of 1.5 kbp and 1.0 kbp markers indicated.
