Table 1 Comparison of the success rate of transfection of P. falciparum by LyRET, Epreloading, and EiRBC methods.
From: Lyse-Reseal Erythrocytes for Transfection of Plasmodium falciparum
Plasmids | Transfection methods used for P. falciparum 3D7 | |||||
|---|---|---|---|---|---|---|
LyRET | EiRBC | Epreloading | ||||
No. of successful transfections/total No. of transfections done | Days to emergence of resistant parasites | No. of successful transfections/total No. of transfections done | Days to emergence of resistant parasites | No. of successful transfections/total No. of transfections done | Days to emergence of resistant parasites | |
pPfCENv3 | 8/8 | 11 ± 0.9 | 5/5 | 11.4 ± 0.5 | 3/4 | 14 ± 0 |
HFDDI | 7/8 | 37.3 ± 5.8 | 4/4 | 26.5 ± 3.5 | *NA | NA |
pFCEN1 | 1/2 | 46 | 2/2 | 23 ± 0 | NA | NA |
Transfection methods used for P. falciparum D10 | ||||||
pPfCENv3 | 6/6 | 14 ± 1.6 | NA | NA | NA | NA |
