Table 2 Optimization of plasmid DNA amount for transfection of P. falciparum 3D7 by the LyRET method.

From: Lyse-Reseal Erythrocytes for Transfection of Plasmodium falciparum

μg of pPfCENv3/transfection

No. of successful transfections/total No. of transfections done

Days to emergence of resistant parasites

0

0/2

—

1

0/2

—

2.5

2/2

24 ± 1.4

5

2/2

23 ± 0

10

6/6

17 ± 4.7

20

4/4

16 ± 1.2

40

4/4

12.5 ± 1.9

80

3/3

13 ± 2

100

4/4

12 ± 2

  1. Each transfection experiment was independently performed. The days to emergence of resistant parasites is mean with SD of the days required for emergence of resistant parasites in successful experiments.
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