Table 3 Transfection of P. falciparum 3D7 with pPfCENv3 using RBCs of different blood groups by the LyRET method.

From: Lyse-Reseal Erythrocytes for Transfection of Plasmodium falciparum

Blood groups

LyRET

EiRBC

No. of successful transfections/total No. of transfections done

Days to reach 1–2% parasitemia

%GFP positive cells

No. of successful transfections/total No. of transfections done

Days to reach 1–2% parasitemia

%GFP positive cells

O+

4/4

20.0 ± 0.0

53.1 ± 8.5

4/4

19.5 ± 0.7

49.2 ± 6.6

A+

4/4

35.0 ± 9.9

32.2 ± 3.1

*NA

NA

NA

B+

4/4

20.5 ± 0.7

48.3 ± 24.5

NA

NA

NA

AB+

4/4

34.0 ± 11.3

34.3 ± 11.7

NA

NA

NA

  1. Each transfection experiment was independently performed. The days to reach 1–2% parasitemia is mean with SD of the days required for emergence of resistant parasites in successful experiments. The %GFP positive cells was determined by FACS and is mean with SD of GFP-Hoechst positive cells in all the successful experiments. *NA indicates “Not Attempted”.