Figure 3

Results of the thresholding analysis for the different alginate microspheres. (a) A schematic representation of a topography example (left, dark blue) is sectioned (planes depicted in gold) to illustrate thresholding analysis at 10, 20 (for valley analysis), 80 and 90% (for peak analysis) of the maximum image height (MIH). The result of these sections is depicted for a representative image on the right. The areas corresponding to peaks above 80% of the MIH are highlighted in red, as are the areas corresponding to valleys below 20% of the MIH. (b) Mean peak diameter calculated through grain analysis at 80% threshold of the MIH. (c) Mean valley diameter calculated through grain analysis at threshold 20% of the MIH. Xy scale of the thresholded image is 3 × 3 µm, whereas z scale is 74 nm. (d) Total valley volume in absolute value, calculated through grain analysis similarly to (c). n = at least 5 samples per group. All graphs depict mean ± standard error of the mean. One-way ANOVA with Bonferroni post-hoc test. ^#p < 0.05 for APP-GDP comparisons.