Table 3 Conversion and ee values of d-1k,c obtained from the corresponding the ammonia elimination reactions performed under optimal conditionsa.

From: The production of l- and d-phenylalanines using engineered phenylalanine ammonia lyases from Petroselinum crispum

Substrate

Substituent

PcPAL

[S] (mM)

Time (h)

c (%)

eeD (%)

rac-1c

p-CH3

I460V

5

48

~50

95

rac-1k

m-CF3

I460V

2

23

~50

93

  1. aReaction conditions: assays were performed in 1.5 mL polypropylene tubes at 30 °C, 200 rpm for 16 h, in 500 µL reaction volume, using the corresponding substrate concentration, ratio of cell density (OD600)/substrate concentration (mM) of 1.0, and Borax buffer as reaction medium.
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