Figure 3

Cytokine measurements from the spleens of noninfected (a) and L-infantum infected (b) mice after treatment with empty scYCWPs (scYCWPs), T6-loaded scYCWPs (scYCWPs + T6) and T6 alone (T6). Miltefosine was used as the standard drug against visceral leishmaniasis. Data were expressed as cytokine production in pg/ml and shown as mean ± standard error (n = 4). Using the BD Cytometric Bead Array (CBA) Mouse Th1/Th2/Th17 Cytokine Kit manufacturer’s instruction, 2,500 events were analyzed for each sample. Significant differences at *p < 0.05, **p < 0.01, ***p < 0.001.