Figure 5
From: Pilot in vivo studies on transcutaneous boiling histotripsy in porcine liver and kidney
Operating at higher peak power outputs and shorter pulses accelerates BH treatments. Histological slides of the volumetric lesions stained with Masson’s trichrome stain in kidney cortex for low dose BH exposures consisting of 10 pulses of progressively shorter duration (as indicated) delivered at increasing pulse repetition frequency (duty cycle was constant and equal to 1%). As the pulse duration was decreased, peak acoustic output power was increased to achieve boiling within each pulse (Table 1 and Fig. 9). The degree of liquefaction was the same across all exposures, while accelerating the treatment 10-fold. At this lower dose, some connective tissue fragments and structures with adjacent cells (black arrows) were observed within the lesions. The scale bar is 250 microns.
