Figure 7

Effect of CYP2B11 3′-UTR polymorphisms on gene expression. (a) Firefly luciferase 3′-UTR reporter plasmids were constructed using the complete 3′UTR region cloned using DNA from dogs homozygous for the H1, H2 and H3 haplotypes. (b) Plasmids were co-transfected with Renilla luciferase (transfection control) into MDCK cells and assayed using the Dual-Glo assay kit. Results are expressed relative to CYP2B11-3′UTR-H1 plasmid transfected cells and represent the mean (standard deviation) of 3 independent experiments conducted in quadruplicate. Significant differences between haplotypes were identified by ANOVA (P < 0.05). Shown are the P-values for post hoc pairwise multiple comparisons testing to H1 control (Holm-Sidak method).