Table 1 Correlation of CYP isoform protein content with CYP marker activities measured in dog liver microsomes.

From: Pharmacogenomics of poor drug metabolism in Greyhounds: Cytochrome P450 (CYP) 2B11 genetic variation, breed distribution, and functional characterization

Enzyme marker activity

Attributed CYPs

Spearman correlation coefficient (P-value)

Human

Dog

CYP1A protein

CYP2B11 protein

CYP3A protein

Phenacetin-o-deethylation

CYP1A2

CYP1A2

0.59 (0.02*)

0.30 (0.28)

0.10 (0.71)

Coumarin 7-hydroxylation

CYP2A6

CYP2A13

−0.29 (0.29)

−0.35 (0.20)

0.06 (0.83)

Propofol 4-hydroxylation

CYP2B6

CYP2B11

0.32 (0.24)

0.70 (0.003*)

0.18 (0.51)

Bupropion 6-hydroxylation

CYP2B6

CYP2B11

0.25 (0.36)

0.73 (0.002*)

0.00 (1.00)

Flurbiprofen hydroxylation

CYP2C9

?

0.41 (0.13)

0.42 (0.12)

0.14 (0.61)

S-mephenytoin 4-hydroxylation

CYP2C19

?

0.38 (0.16)

0.28 (0.31)

0.15 (0.56)

Dextromethorphan o-demethylation

CYP2D6

CYP2D15

−0.31 (0.26)

−0.47 (0.07)

−0.05 (0.84)

Chlorzoxazone 6-hydroxylation

CYP2E1

CYP2E1

0.06 (0.81)

−0.10 (0.71)

−0.04 (0.87)

Omeprazole sulfonation

CYP3A4

CYP3A12

0.13 (0.64)

0.35 (0.20)

0.86 (<0.001*)

  1. CYP isoform protein content (determined by immunoblotting) and CYP marker activities were measured in the same set of dog liver microsomes (n = 15) and correlated. Shown are the Spearman correlation coefficients and associated P-values (*P < 0.05). Also shown are the human and canine CYP isoforms that have been attributed to each activity by reaction phenotyping. (?) – No evidence yet to identify the dog CYP isoform responsible for this activity.
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