Figure 6

Combination therapy with anti–CTLA-4 and probiotic lysates enhances anti-tumor responses in tumor-draining mesenteric lymph nodes of the CRC model mice. After therapy, the cells in mesenteric lymph nodes (mLNs) of each mouse were isolated, respectively stained with a panel of CD4/CD8/CD25/Foxp3 (for Treg cells, A and B) CD8/CD44/CD62L (for memory CD8 + T cells, D and E), or CD11b/F4/80/CD206 (M2 macrophage cells, G and H), and assessed by flow cytometry. (C, F, and I) The right scatter charts represent the statistical data shown in the left plot. Data are represented as the mean of each group ± SD.