Figure 7

Analysis of tumor-immunity-associated cytokine expression by RT-PCR and ELISA. Total RNA was extracted from peritoneal macrophages (A–D) and mLNs (E–H) of each group to quantify the mRNA expression levels of IFN-γ, TNF-α, IL-2, and IL-10. (I) ELISA analysis of IFN-γ, TNF-α, and IL-2 in serum. Data are represented as the means of each group ± SD of three independent experiments.