Figure 3

Knock-down of BLMH results in delayed wound healing. (a,b) Scratch wound assays were performed on monolayers of siRNA-transfected HaCaT cells and the wound area was analysed after 24 and 40 hours in presence or absence of additional TNFα (5 ng/ml) (n = 6, paired two-tailed Student’s t-tests). Scale bar shows 200 µm. (c,d) Supernatants were collected from the scratch wounds after 40 hours and the levels of CXCL8 and GROα were determined using ELISAs (n = 5, one-way ANOVAs with Sidak’s multiple comparisons test). All values represent individual experiments with mean ± standard deviation. Significant P-values are presented as *p < 0.05; **p < 0.01; ***p < 0.001 and ****p < 0.0001.