Figure 4
From: IL-33/ST2 axis promotes glioblastoma cell invasion by accumulating tenascin-C
IL-33 promotes TNC expression through the NFκB signalling pathway. U251 glioma cells were pretreated with 10 μM LY294002 (PI3K/AKT inhibitor), 35 μM PD98059 (MAPK/ERK), or 10 μM BAY11-7085 (NF-κB) for 60 min before IL-33 treatment (50 ng/ml). Subsequently, (A) TNC mRNA levels were measured by RT-PCR (4 h later), and (B) protein levels were measured by WB (6 h later). Data are shown as the mean ± SEM; n = 5, NS = not significant, *p < 0.05, **p < 0.01, ***p < 0.001.
