Table 2 Bacteriophage API480 proteins identified by ESI-MS/MS, after denaturation and phage particle fractionation on SDS-PAGE gel. Identified phage proteins are listed below. SDS-PAGE gel band in which the proteins were identified have been indicated as well as the protein mass, the number of identified unique peptides and the protein sequence that is covered by the peptide (in %).
From: Characterization of a new podovirus infecting Paenibacillus larvae
Protein | Identified Function | Band no. (most abundant) | Protein MW (kDa) | No. of unique peptides | Sequence coverage (%) |
|---|---|---|---|---|---|
gp1 | 11,12 (11) | 16.55 | 6 | 50.40 | |
gp2 | 11,12 (12) | 9.96 | 4 | 58.30 | |
gp3 | 5,12 (12) | 11.95 | 6 | 55.90 | |
gp4 | Terminase large subunit | 1,2,3,4,5,6,8,9,10,11 (1) | 55.47 | 14 | 36.66 |
gp5 | 1,2,5,10,11,12 (12) | 7.05 | 3 | 38.10 | |
gp6 | Portal protein | 1,2,3,4,5,6,7,8,9,10,11,12 (3) | 68.14 | 35 | 62.68 |
gp7 | 1,2,3,4,5,6,7,8,9,10,11,12 (6) | 31.45 | 31 | 93.70 | |
gp8 | Major capsid protein | 1,2,3,4,5,6,7,8,9,10,11,12 (7) | 34.38 | 34 | 89.16 |
gp9 | 5,9,12,13 (12) | 9.88 | 7 | 67.80 | |
gp10 | 11,12,13 (12) | 14.40 | 5 | 67.50 | |
gp11 | 1,2,3,4,12(3) | 75.23 | 12 | 24.26 | |
gp12 | 1,2,3,4,5,6,8,9,11 (5) | 43.56 | 17 | 50.75 | |
gp13 | 12 | 11.64 | 7 | 68.00 | |
gp14 | 2,6,7,8,9,10,11 (6) | 35.47 | 18 | 55.71 | |
gp15 | 6,7,8,9,10,11,12 (6) | 37.05 | 19 | 63.00 | |
gp16 | 1,2,3,4,5,6,7,8,9,10,11,12 (2) | 128.32 | 71 | 60.75 | |
gp17 | Holin | 12 | 9.95 | 4 | 35.00 |
gp20 | 12 | 12.52 | 3 | 42.90 | |
gp21 | Holin | 12 | 8.27 | 1 | 26.60 |
gp24 | DNA binding protein | 1,2,6,9,12 (1) | 36.60 | 5 | 15.58 |
gp26 | mRNA-degrading endonuclease | 12 | 12.52 | 3 | 28.40 |
gp30 | 11 | 14.13 | 1 | 13.70 | |
gp34 | 12 | 9.76 | 1 | 16.30 | |
gp36 | 10 | 14.43 | 1 | 7.94 | |
gp37 | 12 | 10.62 | 3 | 59.40 | |
gp38 | 12 | 12.33 | 2 | 37.40 | |
gp39 | Deoxyuridine 5′-triphosphate nucleotidohydrolase | 1,2,3,4,5,6,8,9,10,12 (10) | 18.21 | 7 | 49.69 |
gp40 | 10,11,12 (12) | 9.82 | 6 | 66.30 | |
gp43 | 12 | 7.15 | 5 | 87.90 | |
gp44 | Resolvase | 1,2,3,4,5,6,7,8,9,10,11,12 (11) | 19.12 | 8 | 56.90 |
gp46 | DNA polymerase I | 1,2,3,4 (3) | 87.14 | 28 | 42.96 |
gp47 | DNA primase | 1,2,3 (1) | 105.39 | 9 | 12.50 |
gp48 | Dingle-stranded DNA-binding protein | 3,5,8,9,10,11,12 (10) | 17.60 | 11 | 62.10 |
gp49 | 1,8,9,10,11,12 (9) | 20.18 | 13 | 69.48 | |
gp50 | Host-nuclease inhibitor | 8,9,10 (8) | 21,75 | 10 | 60.99 |
gp53 | 11,12 (12) | 8.58 | 1 | 18.40 | |
gp54 | 12 | 11.83 | 3 | 41.20 | |
gp55 | 12 | 6.91 | 2 | 53.30 | |
gp57 | DEAD/DEAH box helicase | 1,2,4,8 (1) | 60.63 | 7 | 20.10 |
gp59 | 8 | 25,95 | 7 | 44.70 | |
gp60 | 11,12 (11) | 19.76 | 3 | 18.00 | |
gp63 | 12 | 9.65 | 2 | 31.30 | |
gp64 | 12 | 7.95 | 2 | 37.30 | |
gp65 | 1,11,12 (11) | 15.46 | 6 | 65.39 | |
gp70 | Antirestriction protein ArdA | 9 | 18,92 | 3 | 19.90 |
gp76 | 1,11,12 (11) | 17.36 | 7 | 59.60 | |
gp77 | 12,13 (12) | 8.14 | 4 | 63.40 |
