Figure 1
From: KCTD15 is overexpressed in human childhood B-cell acute lymphoid leukemia
KCTD15 is up-regulated in common B-cell acute leukemia. (a) Volcano plot displaying 16976 filtered genes between peripheral blood B cells from 3 healthy subjects and leukemic cells from 3 patients affected by B-ALL. The adjusted p-value is calculated by the Benjamini and Hochberg’s algorithm. Red dots represent differential expressed genes; these are in total 683 genes identified from the overlap of two different statistical methods (NoiSeqBio and DESeq. 2) with a threshold (red vertical line) at |log2FC| ≥ 2; the green dot highlights KCTD15 gene. (b) KCTD15 mRNA expression levels in a group of 10 common B-ALL patients at diagnosis (coded as PA-#) and day +33 after therapy (coded as PA-#-R). The relative expression was determined using the 2−ΔCt method. KCTD15 relative expression is shown as mean +/− SD of two technical independent experiments. (c) KCTD15 levels were plotted according to the relative expression measured in BM cells from patients at diagnosis (grey circles) and after therapy (black circles) at day + 33. Transcription of KCTD15 significantly decreased from a median value of 1.25 × 10−3 to 3.38 × 10−4. (**p = 0.002, Wilcoxon matched-pairs test). The inset reports the statistical parameters for the two ensembles.
