Figure 3

Multiparametric FlowCytoMetry (FCM) analysis of pediatric B-ALL bone marrow. Surface (for CD45 and CD19) and intracellular (KCTD15) staining are reported for 5 exemplificative cases of pediatric B-ALL BM samples. Leukemia B-cells (red rectangle) are immunophenotypically detectable by means of intermediate/low expression of CD45 antigen (Column 1) and intermediate expression of CD19 (Column 2 - CD45^dim/CD19^dim). Normal resting lymphocytes (black rectangle) display bright CD45 expression and low CD19 intensity (CD45^bright/CD19^dim). KCTD15 is brightly expressed in both types of cells (Column 3). PA-# = Patient-# at diagnosis.