Figure 5
From: KCTD15 is overexpressed in human childhood B-cell acute lymphoid leukemia
KCTD15 silencing causes leukemic cell death. (a) Bar-plot showing downregulation of KCTD15 mRNA levels in 2′F-ANA KCTD15 treated cells (black bar) compared to 2′F-ANA Scramble (grey dotted-bar) at day +8 and +16 and PBMC (black dotted bar). The relative expression was determined using the 2−ΔCt method and it is shown as mean +/− SD of two technical independent experiments. (b) Western Blot analysis of KCTD15 protein in untreated RS4;11 and 2′F-ANA Scramble and 2′F-ANA KCTD15 treated cells at day +8 and +16. Numbers represent the molecular weight of the protein marker expressed in kDa. (c) Time course analysis of KCTD15 intracellular levels (expressed as % of positive cells) by flow cytometry in RS4;11 cell line treated with 2′-F-ANA Scramble and 2′-F-ANA KCTD15 at day +8 and +16. (d) Annexin-V vs Propidium Iodide Density-plots for 2′F-ANA Scramble and 2′F-ANA KCTD15 treated RS4;11 cell lines at day 8 and 16, respectively.
