Figure 6

KCTD15 upregulation following to PWM stimulation. (a) FSC vs SSC dot-plot for the selection of live cells, the percentage is determined on the total of acquired events. (b) FSC-Height vs FSC-Area dot-plot used for the selection of single cells and doublets exclusion. (c) Dot-plot showing the FSC-height and Cell Tracer Violet intensity in unstimulated PBMC. (d) Dot-plot analysis of showing the FSC-height and Cell Tracer Violet intensity in PBMC stimulated for 5 days with PWM mitogen. The right side of the dot-plot shows resting cells with bright cell tracer fluorescence and low forward scatter, the left side displays active duplicating lymphocytes due to diminished cell trace fluorescence and increased FSC. (e) Dot-plot showing FL1 signal due to the unbound FITC conjugated secondary antibody versus cell tracer violet. (f) Dot-plot showing anti-KCTD15 FITC fluorescence versus cell tracer violet. (g) Forward scatter overlay histogram between Cell Tracer ^Bright/KCTD15^low (green), Cell Tracer ^Bright/KCTD15^high (red) and Cell Tracer ^low/KCTD15^bright subsets. The overlay highlight the increasing FSC signal in KCTD15 cells due to PWM induced activation and proliferation. Numbers represent the percentage of gated cells.