Figure 2 | Scientific Reports

Figure 2

From: An optimized, robust and reproducible protocol to generate well-differentiated primary nasal epithelial models from extremely premature infants

Figure 2

Comparison of culture morphology at ALI using confocal microscopy. (A) Fully differentiated ALI cultures from adults (n = 3, left panel) and extremely premature infants (n = 3, right panel) were assessed for differentiation and stratification using immunefluorescence. Representative 3D reconstructions from adult and preterm models (green = α-tubulin, blue = DAPI, white = Phalloidin, red = MUC5A). Scale bars: 15 µm. (B) Plain view with 63x magnification on the apical cell culture side depicting cilia (green = α-tubulin) and goblet cells (red = MUC5A). Nuclei and actin were counterstained with DAPI and Phalloidin, respectively. (C) Tight junctions were visualized utilizing ZO-1 staining (red). In (B,C), scale bars indicate 10 µm. All confocal images were obtained with an LSM700 confocal microscopy (Carl Zeiss) and images were processed using the Imaris imaging software (Bitplane).

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