Figure 3

Ultrastructural assessment of ALI cultures using transmission electron microscopy. Fully differentiated ALI cultures from healthy adults (n = 3; left panel) and preterm infants (n = 3; right panel) were assessed for ultrastructural properties of goblet cells (A; scale bar = 2 µm), cilia (B; scale bar = 500 nm; scale bar inset = 100 nm) and tight junction complexes (C; scale bar = 500 nm). Representative images are shown. Pictures were acquired with a FEI Tecnai20 electron microscope and processed using Adobe Photoshop.