Figure 1 | Scientific Reports

Figure 1

From: The nuclear receptor FXR inhibits Glucagon-Like Peptide-1 secretion in response to microbiota-derived Short-Chain Fatty Acids

Figure 1

FXR regulates GLP-1 secretion in response to SCFAs ex vivo in the murine colon. (a) Active GLP-1 was measured in supernatants of colonic biopsies from WT mice 5 day-treated with vehicle or GW4064 (30 mg/kg), stimulated with control medium or medium plus Butyrate (1 mmol/l). Data are presented as mean ± SEM (white bars for vehicle-treated mice and grey bars for GW4064-treated mice). (n = 4 mice per group with 3 colonic biopsies per mouse and per stimulation condition). Two-way ANOVA followed by Bonferronni’s post hoc test. *p < 0.05 **p < 0.01. (b) Active GLP-1 was measured in supernatants of WT and FXR KO colonoids stimulated for 2 h with control buffer or buffer plus SCFA mix (acetate 5 mmol/l, propionate 1 mmol/l and butyrate 1 mmol/l). Fold induction compared to WT control condition which was set at 1 (absolute values (mean ± SD) of GLP-1 in the control condition: 0.07 ± 0.09 fmol/µg cell proteins). Data are presented as mean ± SEM of two independent experiments (white bars for WT colonoids and hatched bars for FXR KO colonoids). Two-way ANOVA followed by Bonferronni’s post hoc test. **p < 0.01 ***p < 0.001.

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