Figure 2 | Scientific Reports

Figure 2

From: Characterization and engineering of broadly reactive monoclonal antibody against hepatitis B virus X protein that blocks its interaction with DDB1

Figure 2

Epitope mapping of broadly reactive anti-HBx mAb 2A7. (A) Serially overlapping biotinylated peptides encompassing full BPS HBx length were captured onto Streptavidin-coated plates and subjected to ELISA using 2A7. Optical densities at 450 nm (OD450) were measured and plotted. Start and end positions of each peptide are indicated. (B) Plasmids expressing FLAG-tagged BPS HBx with serial 3 amino acid residues mutated to 3 Ala as indicated were transfected into HEK293T. (C) Plasmids expressing FLAG-tagged BPS HBx with indicated single residue mutation were transfected into HEK293T. Transfected cells were analyzed in Western blot using 2A7 and FLAG antibody. Densitometry scanning of the blots was performed, and 2A7-generated signals were first adjusted against anti-FLAG-generated signals from the same sample, followed by normalization against BPS HBx. Normalized values are indicated as percentages under each lane.

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