Figure 1
cFP induces the expression of LeuO via the membrane sensor protein ToxR. (a) β-Galactosidase activity of a leuO-lacZ fusion in the absence (open bars) and presence (solid bars) of exogenous 5 mM cFP in wild type V. vulnificus, a toxR-deletion isotype, and the deletion mutant supplemented with toxR in trans (pBBR12-toxR). The error bars denote standard deviations of the results of three independent experiments (**P < 0.005). (b) Western hybridization using a polyclonal antibody against LeuO of total proteins from wild type V. vulnificus, toxR-deletion, and the toxR-deletion mutant supplemented with toxR in trans (pBBR12-toxR) grown in the absence and the presence of cFP.
