Figure 2 | Scientific Reports

Figure 2

From: The Influence of Cell Culture Density on the Cytotoxicity of Adipose-Derived Stem Cells Induced by L-Ascorbic Acid-2-Phosphate

Figure 2

Different cytotoxic effects of A2-P and ascorbic acid exerted on ASCs. Viability of ASCs was measured at different cell seeding densities and exposed to various concentrations of A2-P or ascorbic acid for 4 and 7 days. The activity index of ASCs was defined as a ratio of fluorescent value of alamar blue relative to that of day 1. Overall, cell viability was pronouncedly lower in the ascorbic acid group relative to the A2-P group at the same concentration. Increasing concentrations of A2-P and ascorbic acid both decreased the viability of ASCs at the same seeding density. Data are presented as mean ± SD of 3 independent experiments. *p < 0.05, ** P < 0.01, ***P < 0.001 relative to the 0 μM group of respective seeding densities.

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