Figure 4

Association between ASC size and A2-P-induced cytotoxicity. (a) Measurement of microscopic images showed significant smaller cell size of spheroid-derived ASCs relative to ASCs. **P < 0.01; Scale bar = 20 μm. (b) Spheroid-derived ASCs have significantly smaller area of spread compared to ASCs. **P < 0.01; Scale bar = 20 μm. (c) Viability of ASCs and spheroid-derived ASCs were evaluated by alamar blue assay at different seeding densities and various A2-P concentrations for 4 days. Spheroid-derived ASCs showed significantly lower tolerance than ASCs to A2-P-induced cytotoxicity. Data are presented as mean ± SD of 3 independent experiments. *p < 0.05, **P < 0.01, ***P < 0.001 relative to the ASC group of respective A2-P concentrations.