Figure 5
Expression of antioxidant enzymes in ASCs, spheroid-derived ASCs, and fibroblasts at low (L: 2500 cells/cm2) and high (H: 10000 cells/cm2) seeding densities. (a) Real-time PCR measurements for antioxidant enzymes catalase, SOD1, and SOD2 of ASCs, spheroid-derived ASCs, and fibroblasts. ASCs exhibited significant upregulation of all three antioxidant enzymes catalase, SOD1, and SOD2 when seeded at high density. Data are presented as mean ± SD of 3 independent experiments. *p < 0.05, **P < 0.01 relative to low-density culture condition. (b) Representative western blot analysis of catalase, SOD1, and SOD2 protein expression in ASCs, spheroid-derived ASCs, and fibroblasts cropped from different parts of the same gel. Full length blot is presented in Supplementary Fig. 1.
