Figure 6
Influence of an additional antioxidant on A2-P-induced cytotoxicity. (a) ASCs were seeded at density of 10,000/cm2 and 250 μM A2-P was supplemented with or without 200 U/ml catalase for 48 h. In another group, ASCs were treated with the catalase inhibitor 20 mM 3-AT before exposing to 250 μM A2-P for 48 h. Relative viability of ASCs was estimated by alamar blue assay. Co-administration of catalase significantly increased cell viability, while pre-treatment of 3-AT decreased ASC viability compared to the A2-P-only group. **P < 0.01, ***P < 0.001. (b) Light microscopic images of ASCs cultured at different densities under 500 μM A2-P with or without 3 mM NAC, a ROS inhibitor. Treatment of NAC appeared to reverse the cytotoxic effect of A2-P. Scale Bar = 300 μm. (c) Viability of ASCs were evaluated by alamar blue assay at 1250, 2500, 5000, 10000 cells/cm2 with treatment of different concentrations of A2-P with or without 3 mM NAC. Co-administration of NAC reverted the decreased cell viability of A2-P across all A2-P concentrations and seeding densities. Data are presented as mean ± SD of 3 independent experiments. *p < 0.05, **P < 0.01, ***P < 0.001 relative to the A2-P group of respective concentrations.
