Figure 3
Ahr deletion in GCPs exacerbates tumorigenesis in a mouse model of SHH medulloblastoma. (a) Kaplan-Meier survival curves for Math1cre; Ptch1f/f (Ptch1 cKO; n = 19) and Math1cre; Ahrf/f; Ptch1f/f (Ptch1 cKO; Ahr cKO; n = 15) mice. (b) Representative haematoxylin/eosin (H&E) staining showing classic histology in both Ptch1 cKO and Ptch1 cKO; Ahr cKO medulloblastomas. (c) Immunohistochemical staining for mitotic marker pH3B (phosphorylated histone H3B) in brown. (d) Quantification of pH3B+ cells/mm2 of tumour (4 non-adjacent sections from 3 tumours of each genotype). (e) Immunostaining for neural differentiation markers synaptophysin, Map2 and NeuN (brown). Statistical comparison of survival curves in panel (a) was performed by log-rank test (p < 0.001 (***)). Data shown is of a single tumor assessed from each animal (n = 3 WT and 3 cKOs), as each animal presented with a single large tumour. Proliferation data in panel (d) was analyzed by Student’s t test (p < 0.05 (*)). Bars represent mean values +/− SEM. Scale bars: 50 um.
