Figure 4
SMAD3 phosphorylation is increased in Ahr-deficient SHH medulloblastomas. (a) Western blots of total cell lysates of isolated end stage medulloblastoma tissue with antibodies specific to phosphorylated S423/S425 residues of SMAD3 (P-SMAD3), total SMAD2/3 and GAPDH proteins. (b) Quantification of band optic density for phosphorylated SMAD3 relative to SMAD2/3 levels, normalized to GAPDH levels. Data is representative of 3 animals/genotype. (c) Immunohistochemical staining of end stage tumour sections with P-SMAD3 antibody (in brown) with methyl green counterstaining nuclei. (d) Quantification of P-SMAD3+ cells/mm2 of tumour tissue (4 non-adjacent sections from 3 tumours of each genotype). Data was analyzed by Student’s t test (p < 0.001 (***)). Bars represent mean values +/− SEM. Scale bars: 100 um.
