Figure 5
From: Epigenetic reprogramming underlies efficacy of DNA demethylation therapy in osteosarcomas
Molecular evolution of OS based upon DNA methylation (A) and gene mutations (B). (A) Shared and unique DNA methylation between three pairs of primary and metastatic tumors was analyzed. We analyzed 4,327 genes (i) whose TSS200 CGIs were unmethylated in the normal tissue and methylated in OS, and (ii) which were expressed in mesenchymal stem cells and osteoblasts. Two metastatic tumors (Patients #8 and #16) showed a pattern of clonal evolution, one tumor from Patient #21 showed a pattern of parallel evolution. (B) Genomic alterations using NCC Oncopanel v4 between three pairs of primary and metastatic tumors were analyzed. This genetic analysis did not reveal the evolutionary process of distant metastasis.
