Figure 3
From: Regulatory Role of PlaR (YiaJ) for Plant Utilization in Escherichia coli K-12
Confirmation of PlaR-binding in vitro to the regulatory targets: Gel shift assay. Purified PlaR was mixed with 0.5 pM of each DNA probe corresponding to the PlaR-binding regions shown in Fig. 2. PlaR (μM) was added: lane 1, 0; lane 2, 1; lane 3, 2.5; lane 4, 5. Filled triangles indicate the PlaR-DNA probe complex, and arrows indicates free probe.
