Figure 5
From: Regulatory Role of PlaR (YiaJ) for Plant Utilization in Escherichia coli K-12
Influence of PlaR on transcription of the regulatory target genes. E. coli wild-type BW25113 (lane 1) and its plaR-defective mutant (lane 2) and kdgR-defective mutant (lane 3) were grown in M9-casamino acids (0.5%) medium at 37 °C with shaking under aerobic conditions. In the middle of exponential phase, total RNA was extracted from each culture and subjected to Northern blot analysis. DIG-labelled hybridization probes are shown on the left side of each panel. The amounts of total RNA analyzed were determined by measuring the intensity of ribosomal RNAs.
